Journal: International Microbiology
Article Title: An insight into differential protein abundance throughout Leishmania donovani promastigote growth and differentiation
doi: 10.1007/s10123-022-00259-4
Figure Lengend Snippet: 2DE of total protein extracts from L. donovani promastigotes in early logarithmic phase (day 2). IEF was performed in a non-linear 3–10 pH interval. The 2DE gel represents one out of three biological replicates and was stained with SYPRO Ruby. The image was analyzed with PDQuest 2D Advanced 8.0.1 software together with all others, generating a master gel composite image. Most spots were automatically recognized. All spots were manually curated. The spots that were not automatically recognized were manually curated and included in analysis when the 3D intensity graph showed a 3D gaussian, Poisson, hypergeometric, or bimodal distribution. Those showing an irregular intensity distribution were considered noise and were not included in analysis. The spots showing this pattern that had been automatically recognized by the software were removed from analysis. 176 spots were recognized in this gel as a result of the manual curation process
Article Snippet: After staining with SYPRO Ruby (BioRad), the images were analyzed with PDQuest 2D Advanced 8.0.1 software (BioRad) according to the manufacturer’s instructions.
Techniques: Staining, Software